Does anyone has the same situation?
After I sonic my sample and centrifuge them , aliquot them , put them -80c, this morning , when I dissove them on ice , they all have the precipitate.I know it bz the SDS in it, but do I need warm them to let tehm clear or centrifuge again?
I need to continue IP.
Thanks thousand times!
wll
0
2 replies to this topic
#2
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Epigenetist
Posted 28 March 2005 - 10:49 AM
Don't worry. That's normal and it's OK to store samples after sonication.
#3
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Veteran
Posted 28 March 2005 - 02:39 PM
If you let the sample warm to room temp, the precipitate should dissolve away again, although not doing this step has not affected my IP's in the past.
Nick
Nick
Edited by methylnick, 28 March 2005 - 02:40 PM.
