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ChIP sample storage


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#1 wllway

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Posted 28 March 2005 - 10:17 AM

Does anyone has the same situation?
After I sonic my sample and centrifuge them , aliquot them , put them -80c, this morning , when I dissove them on ice , they all have the precipitate.I know it bz the SDS in it, but do I need warm them to let tehm clear or centrifuge again?
I need to continue IP.
Thanks thousand times!
wll

#2 pcrman

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Posted 28 March 2005 - 10:49 AM

Don't worry. That's normal and it's OK to store samples after sonication.

#3 methylnick

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Posted 28 March 2005 - 02:39 PM

If you let the sample warm to room temp, the precipitate should dissolve away again, although not doing this step has not affected my IP's in the past.

Nick

Edited by methylnick, 28 March 2005 - 02:40 PM.





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