DEPC-water and DEPC-PBS
Posted 26 March 2005 - 11:05 PM
1. clean and autoclave the empty bottles
2. fill with 1 L NANO water and add PBS tablets for DEPC-PBS
3. add 1 ml DEPC to DEPC-water and DEPC-PBS respectively
4. shake the bottles at 250rpm at 37C O/N
Do you guys do the same, especially autoclave the bottles first before filling with NANO-water? I have this question because some of my labmates argue why we have to autoclave the bottles for two times, but the other labmates say RNA extracted was degraded when they didnt pre-autoclave the bottles. Do you have the same problem??
Posted 28 March 2005 - 07:18 AM
Posted 28 March 2005 - 08:09 AM
in my lab we let DEPC trteatment for at least 24 hours in a non pre autoclaved bottle. And non particualry degradation if extraction done quick and on ice.
Posted 29 March 2005 - 08:07 AM
for the DEPEC treated water of RNA extraction, we put the depec on the nano water then leave them for 24 hours (the water we use is autoclaved before adding the DEPEC)
after adding the DEPEC we leave it for 24 hours then autoclave again
Posted 29 March 2005 - 10:01 PM