detection of protein phosphorylation using M.S.
Posted 25 March 2005 - 09:01 PM
Posted 26 March 2005 - 08:14 AM
Posted 26 March 2005 - 01:43 PM
I guess my question is what if I don't know what I am looking at or I want to look at all the molecules along a signaling pathway. Can I load samples from a mixture of many different proteins (after digestion) to m.s. and identify their phosphorylation state? If so, it would be a lot more efficient than other methods like WB, FACS and microarray.
Posted 29 March 2005 - 12:30 AM
i've done phosphorylation state analysis once, and for analyzing particular protein, i suppose it's better to separate them on gel and after input one by one in the MS. That is what we did...