4 replies to this topic

[flin]

Is it possible to detect protein phosphorylation by mass spec using lysate? If the sample has to be enriched before M.S., what will be the best way to do it?

[leekaming]

Mass spectrometry should be able to identify the difference in MW of phosphorylated and unphosphorylated protein. MALDI-TOF should be able to distinguish difference of 10Da!

[flin]

Thanks for the info!
I guess my question is what if I don't know what I am looking at or I want to look at all the molecules along a signaling pathway. Can I load samples from a mixture of many different proteins (after digestion) to m.s. and identify their phosphorylation state? If so, it would be a lot more efficient than other methods like WB, FACS and microarray.

[fred_33]

hi
i've done phosphorylation state analysis once, and for analyzing particular protein, i suppose it's better to separate them on gel and after input one by one in the MS. That is what we did...

[flin]

Thx!

fred_33, on Mar 29 2005, 01:30 AM, said:

hi
i've done phosphorylation state analysis once, and for analyzing particular protein, i suppose it's better to separate them on gel and after input one by one in the MS. That is what we did...

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