Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Plasmids in eluting buffer


  • Please log in to reply
1 reply to this topic

#1 indoubt

indoubt

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 46 posts
0
Neutral

Posted 24 March 2005 - 08:38 PM

Mini/maxi prep of plasmids are stored in the elution buffer. I wonder when i use these plasmids for further working like sequencing or PCR should i first purify them from this eluting buffer or can i use them directly in this buffer? :o



Thanks.

#2 fred_33

fred_33

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 288 posts
0
Neutral

Posted 25 March 2005 - 04:07 AM

hi
for sequencing plasmids, i purify them and resuspend them in ddH2O. I use an amersham kit for that, but maybe your sequencing facilities are ok when resuspending in TE?

fred




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.