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questions about mycoplasms


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#1 dan_

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Posted 24 March 2005 - 01:38 PM

Hi,

Does somebody knows if mycoplasms are killed by penn/strep in primary human cells culture? Also, I want to know what is the best way to see if I got mycoplasms into my culture?

Thanks a lot

#2 fred_33

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Posted 25 March 2005 - 06:29 AM

hh... mycoplasma aren't killed by peni strepto... that's such a shame. But first you have to diagnose if your cultures are contaminated. A pcr run will do the job. (just pick 100L of supernatant of a culture dish/plate/well and aboil it 5', then PCR.
If you get contaminations, some companies sell mycoplasmas killing facilities, and your hoods are contaminated too... <_<
But honneslty, if your cultures are contaminated, i would throw them away and start with fresh ones.




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