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Soft agar assay


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4 replies to this topic

#1 littlecell

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Posted 24 March 2005 - 08:08 AM

hello, everyone, i would like to do soft agar assay. but i don't where i can get 2X RPMI ? your information will be appreciated. thanks a million.

#2 diana

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Posted 24 March 2005 - 11:39 AM

hello, everyone, i would like to do soft agar assay. but i don't where i can get 2X RPMI ? your information will be appreciated. thanks a million.

<{POST_SNAPBACK}>


I would make it from powder. You'll have to add bicarbonate to it. See GIBCO catalog.

https://catalog.invi...=39&npc=2&nc=3

#3 littlecell

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Posted 24 March 2005 - 11:55 AM

thanks a lot for your information. but i have another question for the RPMI power. for example, if i have a 1X10L RPMI power and dissolve it into 1L water, it should be 10XRPMI media, right? by the way, is the sterile deionized water good to dissolve it for the soft agar assay?
thank you very much!

#4 diana

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Posted 24 March 2005 - 04:31 PM

thanks a lot for your information. but i have another question for the RPMI power. for example, if i have a 1X10L RPMI power and dissolve it into 1L water, it should be 10XRPMI media, right? by the way, is the sterile deionized water good to dissolve it for the soft agar assay?
thank you very much!

<{POST_SNAPBACK}>



Yes, you are right, you'll obtain 10X media. I would use sterile water to dissolve the RPMI powder because I don't want my agar contaminated.

#5 littlecell

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Posted 25 March 2005 - 06:26 AM

Diana, thanks all your trouble and assistance very much!




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