5 replies to this topic

[seasons]

Should i always work my DNA, RNA and oligonucleotides on ice or can i also work with them in room temperatures?  



Thanks.

[fred_33]

hi
ALWAYS work seems quite strong, but i work as many as possible on ice.

[seasons]

fred_33, on Mar 24 2005, 07:27 AM, said:

hi
ALWAYS work seems quite strong, but i work as many as possible on ice.

<{POST_SNAPBACK}>

What is the reasons for that or does this depend on the protocol? The DNA in the cells in our body does not need ice.    

Thanks.

[fred_33]

rnas are very sensitive pieces. So always work in ice with them.

For primers, i think it's better to defrost them slowly on ice to avoid degradaztion. But after, as you'"re relatively quick, no problem to be at RT.

For DNA, it's also quite sensitive material. Hence i thaw it on ice and try to work in ice.
In cells there is plenty of dna repair porteins (why i am not one? ), and dna is well stabilized

[MPK]

Hi,


For DNA and primers, there is no a big problem even if you forget them overnight at RT° once, twice but not forever or often!!!
For RNA, which is more sensitive to be degraded by the omnipresent RNase,  it's necessary to keep them in ice !!


good luck

fred_33, on Mar 24 2005, 10:13 AM, said:

rnas are very sensitive pieces. So always work in ice with them.

For primers, i think it's better to defrost them slowly on ice to avoid degradaztion. But after, as you'"re relatively quick, no problem to be at RT.

For DNA, it's also quite sensitive material. Hence i thaw it on ice and try to work in ice.
In cells there is plenty of dna repair porteins (why i am not one? ), and dna is well stabilized

<{POST_SNAPBACK}>

for

[lula]

hi
its better to work on ice specially i u r  alow worker and take alot of Time to prepare PCR mixtures
u can leave primers to thaw slowely on ice ,,,and work with them in room temperature then freez them again ,but u better avoid this in RNA as its very sensetive

Edited by lula, 31 March 2005 - 10:35 AM.