Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Working with DNA, RNA and oligonucleotides


  • Please log in to reply
5 replies to this topic

#1 seasons

seasons

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 30 posts
0
Neutral

Posted 24 March 2005 - 04:56 AM

Should i always work my DNA, RNA and oligonucleotides on ice or can i also work with them in room temperatures? :)



Thanks.

#2 fred_33

fred_33

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 288 posts
0
Neutral

Posted 24 March 2005 - 06:27 AM

hi
ALWAYS work seems quite strong, but i work as many as possible on ice.

#3 seasons

seasons

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 30 posts
0
Neutral

Posted 24 March 2005 - 07:17 AM

hi
ALWAYS work seems quite strong, but i work as many as possible on ice.

<{POST_SNAPBACK}>

What is the reasons for that or does this depend on the protocol? The DNA in the cells in our body does not need ice. :)

Thanks.

#4 fred_33

fred_33

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 288 posts
0
Neutral

Posted 24 March 2005 - 09:13 AM

rnas are very sensitive pieces. So always work in ice with them.

For primers, i think it's better to defrost them slowly on ice to avoid degradaztion. But after, as you'"re relatively quick, no problem to be at RT.

For DNA, it's also quite sensitive material. Hence i thaw it on ice and try to work in ice.
In cells there is plenty of dna repair porteins (why i am not one? :o), and dna is well stabilized

#5 MPK

MPK

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 27 March 2005 - 06:37 PM

Hi,


For DNA and primers, there is no a big problem even if you forget them overnight at RT° once, twice but not forever or often!!!
For RNA, which is more sensitive to be degraded by the omnipresent RNase, it's necessary to keep them in ice !!


good luck

rnas are very sensitive pieces. So always work in ice with them.

For primers, i think it's better to defrost them slowly on ice to avoid degradaztion. But after, as you'"re relatively quick, no problem to be at RT.

For DNA, it's also quite sensitive material. Hence i thaw it on ice and try to work in ice.
In cells there is plenty of dna repair porteins (why i am not one? :D), and dna is well stabilized

<{POST_SNAPBACK}>


for

#6 lula

lula

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 56 posts
1
Neutral

Posted 31 March 2005 - 10:31 AM

hi
its better to work on ice specially i u r alow worker and take alot of Time to prepare PCR mixtures
u can leave primers to thaw slowely on ice ,,,and work with them in room temperature then freez them again ,but u better avoid this in RNA as its very sensetive

Edited by lula, 31 March 2005 - 10:35 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.