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His-Tag purification using Qiagen Ni-NTA spin column

Started by kooisk, Mar 24 2005 12:41 AM

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#1 kooisk

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Posted 24 March 2005 - 12:41 AM

Hi,
I have problems in purification of my target protein using Qiagen Ni-NTA spin column under denaturing condition. there is an extra band found ard 47 kDa besides my target protein which is around 10 kDa. Can someone please help me? Thank you.

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#2 GoGoalGirl

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Posted 25 March 2005 - 11:08 AM

kooisk, on Mar 24 2005, 01:41 AM, said:

Hi,
I have problems in purification of my target protein using Qiagen Ni-NTA spin column under denaturing condition. there is an extra band found ard 47 kDa besides my target protein which is around 10 kDa. Can someone please help me? Thank you.

<{POST_SNAPBACK}>

Use pH or imidizole gradient elution.

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#3 kooisk

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Posted 26 March 2005 - 03:48 AM

GoGoalGirl, on Mar 25 2005, 12:08 PM, said:

kooisk, on Mar 24 2005, 01:41 AM, said:

Hi,
I have problems in purification of my target protein using Qiagen Ni-NTA spin column under denaturing condition. there is an extra band found ard 47 kDa besides my target protein which is around 10 kDa. Can someone please help me? Thank you.

<{POST_SNAPBACK}>

Use pH or imidizole gradient elution.

<{POST_SNAPBACK}>

Tq.i will try and see.

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