This is what I want in my 10X reaction buffer:
50mM Tris-HCl pH 2.5
I want the total volume of my buffer to be between 3-5mL so if I mess up when using the buffer, I'd still have some left over.
I found the dilution factors for each, so for example, for the Tris-HCl:
1000mM/50mM = 20.
Then I took the total volume/dilution factor to find out how much Tris-HCl I would need: 5mL/20mL = 1/4 mL Tris-HCl
For CaCl2: 1000/10 = 100 fold dilution factor
5mL / 100mL = 1/20mL CaCl2 (same amount for MgCl2)
Then, I added them up and subtracted by 5mL to find how much water I needed to add:
1/4 + 1/20 + 1/20 = 7/20 reaction solutions
5- 7/20 = 93/20 mL H2O
Would doing it this way get me the 10X buffer I need?
Edited by claritylight, 23 March 2005 - 12:34 PM.