Posted 23 March 2005 - 08:59 AM
Posted 23 March 2005 - 12:43 PM
you do not have to balance this gap. these differences are generaly used to test transfection efficiency regarding the same quantity of cells in the plate.
Moreover never balance with empty bluescript. this empty vedctor will also transfect cells. even if i don't know this vector's specificities, isuppose it give an antibiotic resistance). Hence, you won't be able to select cels that get your plasmid of the cells that just get empty bluescript
Posted 24 March 2005 - 08:16 AM
The reason to do this is to get uniformity among your transfections, so everything starts equal....
Posted 25 March 2005 - 01:00 PM
Posted 29 March 2005 - 10:52 AM
Using a non-specific vector can often cause problems - negative interaction between the mulitple promoters and other vector elements. Therefore this is not the perfect solution for every cell / vector combination.
If you don't need to standardise the amount of DNA though, don't use anything