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Posted 23 March 2005 - 08:26 AM
Hi, I have been trying to make a few libraries using pSMART vector for a while now and I keep getting high amounts of vector background. My usual routine is to end repair and phosphorylate my fragments, size select on a gel, purify from the gel (TAE) and ligate to the vector provided by lucigen, but I am still getting an unbelievable amount od vector that appears to be self ligating or some kind of nucleotide contamination. Has anyone else used this kit? had this problem? any ideas? I am at the end of my rope.