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S2 cell expression


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#1 mnqcljsm

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Posted 23 March 2005 - 07:18 AM

Does anyone have any experience with protein expression in s2 cells. If so then could you suggest suitable plasmid vectors and transfection protocols.

Any help will be much appreciated, Thanks.

jon

#2 BryanW

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Posted 25 March 2005 - 12:51 PM

I suppose you mean S2 (Schneider -Drosophila).
We routinely use the DES vectors from Invitrogen, which contain many features to simplify cloning, detection, and purification - see the web page http://www.invitroge...cfm?pageid=9504.

For S2 or other mammalian cell transfection, most labs in our building have switched to GenCarrier-1 (from Epoch Biolabs) based on its outstanding performance - high efficiency, low toxicity, consistency and most important -much cheaper compared to other brands. Good luck.

#3 mnqcljsm

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Posted 28 March 2005 - 11:16 AM

Ok, ill check it out. Thanks

jon




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