Can I use human recombinant TGF-ß (R&D Systems) for experiments with murine cell lines?
It seems everyone does, but I could find any definite answer to this question so far...
I'm working with a murine Langerhans cells line and plan to test their production of and susceptibility to TGF-ß after various treatments.
If anyone has suggestions, hints or experiences ín this field, I'm thankful for any information of concern!
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joy_maf
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Posted 25 March 2005 - 01:48 PM
Recombinant mouse TGF-betas (-1, -2 or -3) are not commercially available apparently due to lack of luck in scale-up production. Fortunately, recombinant human TGF-b was shown to bind rodent receptor with high affinity. Be careful that growth factors (include TGF-
from couple of vendors in our hands showed inconsistency and unstable over time (very much like antibody, you need to shop around for a good one).....We found 2 very good in quality only difference in price -
from R&D Systems (TGF-b1 cat#240-B-002, 2ug for $245).
from Epoch Biolabs (TGF-b1 cat#21-00215, 5ug for $285, we used this one cause it's cheaper).
Good luck!
from couple of vendors in our hands showed inconsistency and unstable over time (very much like antibody, you need to shop around for a good one).....We found 2 very good in quality only difference in price - from R&D Systems (TGF-b1 cat#240-B-002, 2ug for $245).
from Epoch Biolabs (TGF-b1 cat#21-00215, 5ug for $285, we used this one cause it's cheaper).
Good luck!
