2 replies to this topic

[indoubt]

Ideas about these questions, please.

#Why is Poly-A sequence upstream of a reporter gene?
#Isogenic cell lines?
#Null cell lines?

Thanks.  

[fred_33]

hi

poly a sequence upstream a reporter gene is for ensuring the good RNA termination, and translation.
isogenic cell lines are cell lines that have the same genetic background but differs from 1 gene
null cell lines are cells that have been knocked down for a locus.

[indoubt]

fred_33, on Mar 23 2005, 02:27 AM, said:

hi

poly a sequence upstream a reporter gene is for ensuring the good RNA termination, and translation.

<{POST_SNAPBACK}>

Usually the poly A sequence lays downstream of a gene to give it good RNA termination and translation. BUt when it lies upstream is something new . Upstream means before the promoter. How come?