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Ideas about these questions, please.


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#1 indoubt

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Posted 22 March 2005 - 06:36 PM

Ideas about these questions, please.

#Why is Poly-A sequence upstream of a reporter gene?
#Isogenic cell lines?
#Null cell lines?

Thanks. :P

#2 fred_33

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Posted 23 March 2005 - 01:27 AM

hi

poly a sequence upstream a reporter gene is for ensuring the good RNA termination, and translation.
isogenic cell lines are cell lines that have the same genetic background but differs from 1 gene
null cell lines are cells that have been knocked down for a locus.

#3 indoubt

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Posted 23 March 2005 - 03:20 AM

hi

poly a sequence upstream a reporter gene is for ensuring the good RNA termination, and translation.

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Usually the poly A sequence lays downstream of a gene to give it good RNA termination and translation. BUt when it lies upstream is something new . Upstream means before the promoter. How come? :(




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