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more questions about protein expression...


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#1 justwonder

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Posted 22 March 2005 - 04:31 PM

on mammalian cells:

# Methylation usually happens in CG islets. Does it occur most on C or G?


# Why are HGPRT, TK and APRT amplified selection markers while neomycine and hygromycin are not amplified selection markers? Why do we use HAT medium for TK marker? :P


Thanks.

#2 fred_33

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Posted 23 March 2005 - 01:51 AM

hi
will cpg methylase methylates C on cg islands,
human DNA (cytosine 5) methyl transferase (Dnmt1) methylates C on cg island
dcm methylase CCWGG (but i'm not sure of which of the C)

as i found in neb catalog :
alu 1 methlase C in AGCT sequences
bamH1 methylase : C in GGATCC
dam methylase : GATC
EcoR1 methylase : GAATTC
Hae3 methylase : GGCC
hha 1 methylase : GCGC
hpa2 methylase : CCGG
msp1 methylase : CCGG
taq 1 methylase : TGCA

Edited by fred_33, 23 March 2005 - 01:51 AM.





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