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protein expression...

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#1 justwonder



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Posted 22 March 2005 - 04:27 PM

in the mammalian cells:

# Do I need mRNA destabilization signals for my gene expression? If so where should I add it?

# My book says; "cDNA are often obtained by addition of homopolymeric tails into the 5 non coding region which should be removed. Which homopolymeric tails are used for this purpose?

# Which sequences on the 3 end that reduce mRNA stability?

Thanks alot.

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