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linear DNA can be transformed into Ecoli?


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#1 cathy

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Posted 22 March 2005 - 09:52 AM

I am confused about this. linear DNa can not be transformed into E.coli, right?

#2 franklincon

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Posted 22 March 2005 - 12:13 PM

I am confused about this. linear DNa can not be transformed into E.coli, right?

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Surely it can, some of them can integrated into genomic DNA to present the host cell a character of the plasmid, such as anti-anitibiotic activity.

#3 sharath

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Posted 23 March 2005 - 02:39 AM

The nature of the DNA does not affect transformation. For the DNA to be maintained in the cell, it should have a origin of replication to ensure autonomous duplication or should bear a site to permit recombination with the host DNA. Otherwise, the DNA might be lost from the cell druing replication.
Sharath B.




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