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linear DNA can be transformed into Ecoli?
2 replies to this topic
Posted 22 March 2005 - 09:52 AM
I am confused about this. linear DNa can not be transformed into E.coli, right?
Posted 23 March 2005 - 02:39 AM
The nature of the DNA does not affect transformation. For the DNA to be maintained in the cell, it should have a origin of replication to ensure autonomous duplication or should bear a site to permit recombination with the host DNA. Otherwise, the DNA might be lost from the cell druing replication.