2 replies to this topic

[cathy]

I am confused about this. linear DNa can not be transformed into E.coli, right?

[franklincon]

cathy, on Mar 22 2005, 10:52 AM, said:

I am confused about this. linear DNa can not be transformed into E.coli, right?

<{POST_SNAPBACK}>

Surely it can, some of them can integrated into genomic DNA to present the host cell a character of the plasmid, such as anti-anitibiotic activity.

[sharath]

The nature of the DNA does not affect transformation. For the DNA to be maintained  in the cell, it should have a origin of replication to ensure autonomous duplication or should bear a site to permit recombination  with the host DNA. Otherwise, the DNA might be lost from the cell druing replication.