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Side effects due to transfection procedures

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#1 Bomber



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Posted 22 March 2005 - 09:32 AM

Hi everybody,

I have a question concerning transfection procedures:
I transiently transfected Raji cells by electropration and took a few samples for analysis in a western blot.
Surprisingly I noticed a strong decrease of a certain protein in comparison to non-transfected cells which I analysed on a western blot as well (the actin signals are equal).
I donīt really have an explanation for it: does anybody have an idea if this might be due to electroporation side effects, or are their any papers describing such things?

The vector codes for a shRNA: but the decrease of the protein can be detected as well as in my transfected control cells so I donīt think the effects are due to the used plasmids.

Thanks very much everybody and kind regards

#2 Scott



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Posted 23 March 2005 - 05:42 AM

Hi Bomber,

It is not surprising that after electroporation your cells will alter expression of some genes. Passing a large electric shock through them will result in a number of physiological changes. I haven't seen any papers on it, but it is something that must be remembered that a true control is not untransfected it is mock transfected cells.

Sorry I can't be more help


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