I am using T4 ligase from invitrogen. the product manual said after incubation add 1ul 0.5MEDTA to inactivate the ligase. I did not do this before, do you do this inactivation step?
thanks
cathy
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4 replies to this topic
#2
fred_33
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Posted 22 March 2005 - 05:38 AM
hi
i never do this. After ligation i pellet the dna using ethanol/na acetate and electropore directly.
i never do this. After ligation i pellet the dna using ethanol/na acetate and electropore directly.
#3
sharath
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Posted 23 March 2005 - 02:41 AM
I use the ligation mix directly for transformation
Sharath B.
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george@CASE
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Posted 23 March 2005 - 11:55 AM
yep, I just use the ligation reaction for transformation. It has worked for me everytime.
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vetticus3
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Posted 23 March 2005 - 07:07 PM
ethanol precipitate and transform.
