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Using Glycogen as a carrier in DNA Extraction

Started by Miles, Mar 22 2005 01:12 AM

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#1 Miles

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Posted 22 March 2005 - 01:12 AM

I am trying to extract DNA from samples that have a low amount of cells present, and have read in protocols to add glycogen, LPA, tRNA, etc, to improve yields.  How exactly does glycogen work as a carrier in DNA precipitation to give greater yields?

Many thanks

#2 Maria

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Posted 23 March 2005 - 05:11 AM

I'm not 100% sure, guess it binds to the DNA present?

:(

#3 pcrman

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Posted 23 March 2005 - 07:23 AM

check these references:

Tracy S Prep. Biochem 1981; 11:251-268
Helms, C et al DNA 1985: 4 39-49
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