How to make a stable cell line using suspension cells
Posted 21 March 2005 - 06:57 PM
I am going to transfect suspension cells and make a stable cell lines. But I have no experience for this. Could any one teach me that?
Posted 22 March 2005 - 01:23 AM
for transfection of suspension cell line you may see this topics :
well i would say that culturing cells in the media qsupplemented with selection antibiotic would give you good results for establishing cell line.
Here is a protocol that may help you.
best time for adding G418
antibiotics during transfection
tell us if you want more precisions... There are also topics in cell biology forum taht may interest you...
Posted 22 March 2005 - 10:53 AM
Thank you so much for your help. It is really important for me. I will post my result in the near future.
Posted 22 March 2005 - 11:07 AM