Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to make a stable cell line using suspension cells


  • Please log in to reply
4 replies to this topic

#1 popogirlxd

popogirlxd

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 21 March 2005 - 06:57 PM

hi, everyone,
I am going to transfect suspension cells and make a stable cell lines. But I have no experience for this. Could any one teach me that?

thanks !

#2 fred_33

fred_33

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 288 posts
0
Neutral

Posted 22 March 2005 - 01:23 AM

hi
for transfection of suspension cell line you may see this topics :

http://www.protocol-...935

well i would say that culturing cells in the media qsupplemented with selection antibiotic would give you good results for establishing cell line.
Here is a protocol that may help you.

best time for adding G418

antibiotics during transfection

tell us if you want more precisions... There are also topics in cell biology forum taht may interest you...
fred

#3 popogirlxd

popogirlxd

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 22 March 2005 - 10:53 AM

Hi,
Thank you so much for your help. It is really important for me. I will post my result in the near future. :P

#4 popogirlxd

popogirlxd

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 22 March 2005 - 11:07 AM

Thank you for the protocol of selecting transfected cell. But there is a picture in it I can't acess. Is any way I can get it?

Thanks again.

#5 fred_33

fred_33

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 288 posts
0
Neutral

Posted 23 March 2005 - 02:13 AM

hi
:(:(:(
i can't get the picture too...




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.