4 replies to this topic

[popogirlxd]

hi, everyone,
   I am going to  transfect suspension cells and make a stable cell lines. But I have no experience for this. Could any one teach me  that?

thanks !

[fred_33]

hi
for transfection of suspension cell line you may see this topics :

http://www.protocol-...935

well i would say that culturing cells in the media qsupplemented with selection antibiotic would give you good results for establishing cell line.
Here is a protocol that may help you.

best time for adding G418

antibiotics during transfection

tell us if you want more precisions... There are also topics in cell biology forum taht may interest you...
fred

[popogirlxd]

Hi,
Thank you so much for your help. It is really important for me.  I will post  my result in the near future.

[popogirlxd]

Thank you for the protocol of selecting transfected cell. But there is a picture in it I can't acess. Is any way I can get it?

Thanks again.

[fred_33]

hi
:(
i can't get the picture too...