1 reply to this topic

[searcher]

How can i scale up (making a larger culture) from a little bacterial culture?

Thanks.

[Canalon]

searcher, on Mar 21 2005, 05:28 PM, said:

How can i scale up (making a larger culture) from a little bacterial culture?

Thanks.

<{POST_SNAPBACK}>

Easy way prepare a larger flask of fresh medium. Below ~200 ml you can start it with a small amount of bacteria (picked colony, a scratch of a glycerol stock...). Above it it is safer to first start a small preculture (~10ml) and use it to start the bigger culture after one day.

HTH

Patrick