Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Storing of bacterial colonies


  • Please log in to reply
1 reply to this topic

#1 searcher

searcher

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 21 March 2005 - 02:22 PM

How long can i use the bacterial colonies to make new cultures before i trash the agar plate? Where should i store the agar plate with the bacterial colonies?

Thanks for any inputs.

Edited by searcher, 21 March 2005 - 02:24 PM.


#2 fred_33

fred_33

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 288 posts
0
Neutral

Posted 22 March 2005 - 01:26 AM

hi
for making cultures, i use plates for two weeks at most. They are stored at 4C. If it's for minipreps, i do many preps quick till i get my clone. After that, i make a bigger prep and before pellet bacterias i make two aliquots that i store at -80C. (see this topic for freezing thawing bacterias)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.