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Precipitating DNA at low RCF


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#1 AussieUSA

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Posted 21 March 2005 - 01:11 PM

HiYa Fellow BioForumers,

I need to collect plasmid DNA from a large-scale prep but don't have centrifuge tubes for doing > 10,000 rcf ;)

However, I do have access to a centrifuge and tubes capable of 3500 rcf.

My question: how long would I need to spin at 3500 rcf to collect my isopropanol precipitated DNA??

Any assistance would be appreciated :)

Thanks,

#2 methylnick

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Posted 21 March 2005 - 03:04 PM

Hi fellow Aussie,

I spin genomic preps with isopropanol at 3500rcf for 30 minutes. Seems to precipitate fine in my hands. I am sure this will extend to plasmid preps. If you are worried you can always add a co-precipitant such as glycogen to the plasmid prior to centrifugation.

all the best

Nick

All comments and communication are my own personal ones, and are not tied to any of my affiliations. 





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