Precipitating DNA at low RCF
Posted 21 March 2005 - 01:11 PM
I need to collect plasmid DNA from a large-scale prep but don't have centrifuge tubes for doing > 10,000 rcf
However, I do have access to a centrifuge and tubes capable of 3500 rcf.
My question: how long would I need to spin at 3500 rcf to collect my isopropanol precipitated DNA??
Any assistance would be appreciated
Posted 21 March 2005 - 03:04 PM
I spin genomic preps with isopropanol at 3500rcf for 30 minutes. Seems to precipitate fine in my hands. I am sure this will extend to plasmid preps. If you are worried you can always add a co-precipitant such as glycogen to the plasmid prior to centrifugation.
all the best
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