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50% stock cultures


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#1 rosangelafrita

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Posted 21 March 2005 - 09:46 AM

Hi! I normally use glycerol at a final concentration of 50% im my stock cultures (e. coli). i've seen some literature and there's a wide range of the concentration one can use!! anyone knows of any problems in using 50% glycerol concentration?


Thank you in advance

#2 AussieUSA

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Posted 21 March 2005 - 01:16 PM

I have grown bugs from other peoples 50% glycerol stocks with no problem.

I standardly use 200 ul glycerol and 800 ul culture for my glycerol stocks and also have no problem.

Hope this helps.

#3 Canalon

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Posted 22 March 2005 - 01:34 PM

In my lab we regularly use 15% glycerol, it's enough. Increasing the quantity of glycerol do not hurt though.
It is easier to pipet if you prepare a 30% glycerol stock solution in water and add this solution to your bacteria. It is even better if you add somme salts to keep the osmotic pressure OK (~0.8 g/l NaCl final, or 0.4 g/l Sodium phosphate final ).

Patrick




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