50% stock cultures
Posted 21 March 2005 - 09:46 AM
Thank you in advance
Posted 21 March 2005 - 01:16 PM
I standardly use 200 ul glycerol and 800 ul culture for my glycerol stocks and also have no problem.
Hope this helps.
Posted 22 March 2005 - 01:34 PM
It is easier to pipet if you prepare a 30% glycerol stock solution in water and add this solution to your bacteria. It is even better if you add somme salts to keep the osmotic pressure OK (~0.8 g/l NaCl final, or 0.4 g/l Sodium phosphate final ).