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Help:how much gDNA is needed?


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#1 flyinghorse

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Posted 21 March 2005 - 07:54 AM

[B] :(
I want to construct a genomic expression library of a streptococcus spp. But I don't know how much( in ug ) truncated gDNA is needed to construct a representative expression library for immunoscreen. Who can help me?
Thanks a lot!

#2 yongyk

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Posted 21 March 2005 - 09:39 PM

dear flyinghorse,

It really depent on what vector are you using. for me, I use 100ug before and it work.

Good luck
Yongyk




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