I plan on screening a yeast genomic library (YCP50) but have been facing a lot of problems after amplifying the library. My problem is that i can't seem to isolate sufficient amounts of the plasmid for transformation into S. cerevisiae. I've tried the Promega Maxi Prep kit and the Qiagen Mini Prep kit to no avail. I would like to add that the E. coli is an endA+ strain (ie. HB101).
ANY help on this (frustrating) matter will be greatly appreciated.