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[Student54]

Hello,

I develops a protocol of extraction of extracellular proteins of the soil. In a protocol, I saw that one added K2PHO4 in this plug. With what the K2PHO4 is used and it compatible with Tris-HCl and the DTT is?
It does not denature proteins?

Thank's

Edited by Student54, 21 March 2005 - 03:04 AM.

[Simonsays]

Student54, on Mar 21 2005, 04:03 AM, said:

With what the K2PHO4 is used

I believe that HPO4 should have buffering capacities

Simon