How can I thaw competent bacterial cells for transformation? Can i thaw the tube within my hand or would this damage my cells?
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Thawing of competent bacterial cells
Started by justwonder, Mar 20 2005 06:46 PM
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#2
fred_33
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Posted 21 March 2005 - 12:47 AM
hi
it's better to thaw competent bacterias on ice then you strip it on a selective lb agar plate. Then pick one clone, and do the preculture, followed by culture
it's better to thaw competent bacterias on ice then you strip it on a selective lb agar plate. Then pick one clone, and do the preculture, followed by culture
#3
dasgupta_arupratan
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Posted 21 March 2005 - 02:00 AM
thawing the competent cells with hands will definitely harm them. its always better to thaw them by keeping them in ice.....
