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Error-prone PCR


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#1 Barbara

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Posted 07 June 2002 - 01:02 AM

I would like to make a random PCR mutagenesis of cca 1000 bp long DNA fragment.
Could you recommend me any useful protocol or article ?

#2 milanoj

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Posted 07 June 2002 - 05:47 AM

Clontech has a good kit for PCR mutagensis called Diversify. If that's not an option there are many references in PubMed. Try Vartanian J.P. et al. Nuc Acids Res 24(14):2627-2631.
Have fun




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