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[mac1]

I am using biotinylated anti-rat for my secondary antibody and subsequently need to incubate the membrane in ABC for an hour before developing using the ECL. My primary antibody is monoclonal and hence should be very specific.

Question: Anyone out there knows about the likelihood of getting extra band apart from the band of interest. Would the problem be completely eradicated using horse radish peroxidase (HRP) anti-rat as my secondary antibody.

All comments would be welcome.

Cheers