CHIP with nuclei
Posted 18 March 2005 - 06:59 AM
Can CHIP assay be performed using nuclei as its all about chromatin?
Posted 18 March 2005 - 03:11 PM
of course you can perform CHIP with nuclei, though you will have to treat the nuclei by either sonication or micrococcal nuclease digest to fragment the nuclei to a size that can be precipitated with the antibody you use for CHIP.
All comments and communication are my own personal ones, and are not tied to any of my affiliations.