Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

CHIP with nuclei


  • Please log in to reply
1 reply to this topic

#1 nidhi

nidhi

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 18 March 2005 - 06:59 AM

Hello,

Can CHIP assay be performed using nuclei as its all about chromatin?

#2 methylnick

methylnick

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 259 posts
4
Neutral

Posted 18 March 2005 - 03:11 PM

Hi Nidhi,

of course you can perform CHIP with nuclei, though you will have to treat the nuclei by either sonication or micrococcal nuclease digest to fragment the nuclei to a size that can be precipitated with the antibody you use for CHIP.

Good luck!

Nick

All comments and communication are my own personal ones, and are not tied to any of my affiliations. 





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.