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CHIP with nuclei


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#1 nidhi

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Posted 18 March 2005 - 06:59 AM

Hello,

Can CHIP assay be performed using nuclei as its all about chromatin?

#2 methylnick

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Posted 18 March 2005 - 03:11 PM

Hi Nidhi,

of course you can perform CHIP with nuclei, though you will have to treat the nuclei by either sonication or micrococcal nuclease digest to fragment the nuclei to a size that can be precipitated with the antibody you use for CHIP.

Good luck!

Nick




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