DNA pH dependance
Posted 17 March 2005 - 12:38 PM
Posted 17 March 2005 - 02:50 PM
Short answer yes... low pH can de-purinate the DNA expecially if it is single stranded, however I don't know about charge density, from what I remember of DNA structure, most of the reactive groups are involved in the linkages between bases, this is affected by the salt concentration and pH of the solution, but beyond that my chemistry fails me.
I would suggest an organic chemistry text for a more complete answer, or even a literature search on pubmed.
Hope this has helped.
Edited by bob1, 17 March 2005 - 02:50 PM.