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GST tag

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#1 Ell



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Posted 17 March 2005 - 05:16 AM

Hi everybody,
have troubles :rolleyes:... am expressing 50 kDa protein using GST tag(26kDa) and after purification (using Glutathion Sepharose + reduced Glutathion) my protein is 60 kDa (SDS-gel) Really donīt understand it.
Has somebody any idea what could happened or why?
Thanx + Good Luck!

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