Thank you for every one answer me
After RNA extraction by those kit, I try to normal PCR which can see band. Thus, samples were treated with DNaseI and purify by phenol choloform. Next, I try to normal PCR again that can not to see band and then RT-PCR is made. I can not to see band from RT-PCR also. So, I thinks that mRNA is degraded by phenol/choloform method or not. Purification method is not used before RT-PCR, I will success to amplify or not.
Primer is not disized to specific primer but can amplify a gene well from genomic DNA
For inhibitor, I thinks that RNA extraction kit already contained RNase inhibitor. Instument for use in RNA preparation were also double autoclave (tip and microtube) and treated with DEPC (reagent solution).
best my regards
Edited by penguin_th, 17 March 2005 - 05:04 PM.