Posted 17 March 2005 - 12:47 AM
I am dealling with bacteria OMP. I had cloned the OMP gene into QiaExpress vector pQE30 and was N-terminal tagged with His. However, when I purify my protein, I can't get my protein in elution buffer, but they all present in washing buffer. What is happening?
Posted 17 March 2005 - 01:47 AM
i got this problem once and that was a problem of column equilibration...
Posted 22 March 2005 - 12:59 AM
Posted 25 March 2005 - 11:13 AM
Posted 26 March 2005 - 08:42 AM
Posted 27 March 2005 - 06:57 PM
Since I purify my protein in denature condition, will it still form agregate?
If it do form agregate, how to get rid of it?
Posted 28 March 2005 - 02:54 AM