I wish some body can tell me what going on.
I am runing real-time multiplex pcr for subtyping for yeast. I have three probes FAM. Texas Red and Cy5 and there share both forward and reverse primer.
When I run the assay, FAm got very high Fluorescence unit (around 3000), but my Texas Red and Cy5 is like not even 150. How ever their Ct value are around 13-15 cycle.
What determine the Fluorescence unit for real-time assay?
Submit your paper to J Biol Methods today!
real-time multiplex PCR.
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