
I use ABI7500. From the dissociation curve you know the melting point of the PCR products. I perform duplex pcr. So I have two pieks. Can anyone tell me what I am told about the heighs of the pieks?
Thank a lot : )
Ribosoul

Posted 16 March 2005 - 01:16 PM
Posted 16 March 2005 - 07:49 PM
Posted 17 March 2005 - 10:36 AM
Dear Yongyk,Dear Ribosoul,
The height of the piek represent Relative Fluorescence unit (RFU). It tells you qualitatively the amount of the specefic product contain in your tube.
I think the more relevent info getting from the dissasosiation curve is the specific meltting temperature. Dont't worry about the height.
Regards
Yongyk
Posted 17 March 2005 - 12:20 PM
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