coomassie blue stained gel
Posted 16 March 2005 - 12:41 PM
I welcome feedback and comments.
Posted 16 March 2005 - 01:02 PM
Posted 16 March 2005 - 04:08 PM
ohh, does the same hold true for gels stained with Sypro.
The thing is that coomassie and destain contains MeOH and acetic acid which fix the proteins, precipitate them in the gel. The transfer to a membrane becomes then very difficult. You should first transfer and the color the remaining proteins on the gel with Coomassie.
Posted 17 March 2005 - 04:15 AM
SYPRO Tangerine protein gel stain provides fast, easy and sensitive detection of proteins in gels (down to 4 ng/band) without the need for fixatives. Staining is compatible with subsequent Western blotting, zymography, electroelution or mass spectrometry. Compared to Coomassie Brilliant Blue and silver staining, SYPRO Tangerine stain provides more consistent protein-to-protein staining and a much broader linear quantitation range (over three orders of magnitude). Stained proteins can be viewed with a standard UV or blue-light transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, use of the SYPRO protein gel stain photographic filter (S6656) is recommended.