I am trying to make some BL21 AI (arabinose inducable) competent cells to use in protein purification. ( I have a small stock of cells frm a lab we are collaborating with.) The competent cells, according to Invitrogen, have a tetA resistance gene so I can use tet to confirm strain identity. When I streak a small amt of cell stock onto LB/tet 10 ug/mL plates, I get single colonies, but when I seed a single colony from this plate into 2mL LB/Tet 10 media and shake O/N 250 prm 37C, I get no growth! So it seems the colony I picked, even though it was from the Tet plate, is not tet resistant! What am I missing/overlooking here? Thanks.
Tet resistant colonies will not grow in medium
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