2 replies to this topic

[anonymous]

Here I have a basic question, usually, a linearized plasmid will run slower than CCC plasmid after it was digested by a one-cut R.E.But now my linearized vector runs faster than the integrate one after being digested by one -cut-site R.E. I have change two RE, the same case happened ,these two band runs at the same speed.Who can tell me if it could happen in the normal situation?

Thanks in advance.

[anonymous]

Yes, it is quite normal.  You should based on the linearized plasmid to judge the size.  It is very hard to predict the moving length of the uncut plasmid.

[anonymous]

the uncut plasmid also runs as to isoforms, supercoiled and not