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question about plasmid digested by one-cut-site RE


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#1 anonymous

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Posted 17 January 2001 - 10:00 PM

Here I have a basic question, usually, a linearized plasmid will run slower than CCC plasmid after it was digested by a one-cut R.E.But now my linearized vector runs faster than the integrate one after being digested by one -cut-site R.E. I have change two RE, the same case happened ,these two band runs at the same speed.Who can tell me if it could happen in the normal situation?

Thanks in advance.

#2 anonymous

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Posted 17 January 2001 - 10:00 PM

Yes, it is quite normal. You should based on the linearized plasmid to judge the size. It is very hard to predict the moving length of the uncut plasmid.

#3 anonymous

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Posted 18 January 2001 - 10:00 PM

the uncut plasmid also runs as to isoforms, supercoiled and not




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