2 replies to this topic

[LMD2011]

I am doing some stable transfections using linearised plasmid. Each time I linearise the plasmid then purify it prior to transfection. Does anyone know if I can freeze it once linearised ?

[mnqcljsm]

I have frozen linear plasmids before, then successfully ligated them several weeks later after storing at -20. So i guess it works.
Jon

[fred_33]

hi
i do as jon and never get problems.
fred