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2 replies to this topic
Posted 16 March 2005 - 06:16 AM
I am doing some stable transfections using linearised plasmid. Each time I linearise the plasmid then purify it prior to transfection. Does anyone know if I can freeze it once linearised ?
Posted 16 March 2005 - 06:36 AM
I have frozen linear plasmids before, then successfully ligated them several weeks later after storing at -20. So i guess it works.