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Linearised DNA


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#1 LMD2011

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Posted 16 March 2005 - 06:16 AM

I am doing some stable transfections using linearised plasmid. Each time I linearise the plasmid then purify it prior to transfection. Does anyone know if I can freeze it once linearised ?

#2 mnqcljsm

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Posted 16 March 2005 - 06:36 AM

I have frozen linear plasmids before, then successfully ligated them several weeks later after storing at -20. So i guess it works.
Jon

#3 fred_33

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Posted 16 March 2005 - 06:53 AM

hi
i do as jon and never get problems.
fred




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