Hi all,
my question is which is the most successful method for purification of protein with His6 tag?
I have tried with Ni-NTA Spin Kit (QIAGEN) but i'm not so satisfied...i loosed a lot of protein during the purification steps and that (as usual) i received multi band during elution step...
What about magnetic beads?
Thanks in advance!
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#2
fred_33
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Posted 16 March 2005 - 05:09 AM
hi
during a practice, i've sucessfully use beads. It's an easy method which give good results. just swirling and magnetic field....
during a practice, i've sucessfully use beads. It's an easy method which give good results. just swirling and magnetic field....
Edited by fred_33, 16 March 2005 - 05:10 AM.
#3
leekaming
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Posted 27 March 2005 - 09:11 AM
Ni column usually is the preliminary purification step, you can use other column like ion exchange or gel filtration to further purify your protein
