Protein with His6 tag
Posted 16 March 2005 - 03:28 AM
my question is which is the most successful method for purification of protein with His6 tag?
I have tried with Ni-NTA Spin Kit (QIAGEN) but i'm not so satisfied...i loosed a lot of protein during the purification steps and that (as usual) i received multi band during elution step...
What about magnetic beads?
Thanks in advance!
Posted 16 March 2005 - 05:09 AM
during a practice, i've sucessfully use beads. It's an easy method which give good results. just swirling and magnetic field....
Edited by fred_33, 16 March 2005 - 05:10 AM.
Posted 27 March 2005 - 09:11 AM