Do all kidney 293 cells contain an adenovirus? So I can use any 293 cell to amplify a adenoviral stock?
sincerely,
thomas
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#2
fred_33
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Posted 16 March 2005 - 01:40 AM
hi
try a search in the ATCC database. http://www.atcc.org/ (search in cell biology and "name"=293
But you're right, 293 cells (from kidney contains either adenovirus 5 sequnces or SV40 sequences or SV40/CMV...
But for virus amplification, i woul youse Hek 293 PACK cells also known as phoenix helper cells....
Did i answer your question?
fred
try a search in the ATCC database. http://www.atcc.org/ (search in cell biology and "name"=293
But you're right, 293 cells (from kidney contains either adenovirus 5 sequnces or SV40 sequences or SV40/CMV...
But for virus amplification, i woul youse Hek 293 PACK cells also known as phoenix helper cells....
Did i answer your question?
fred
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snowman
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Posted 16 March 2005 - 04:57 AM
hi,
yes, thank you. will the pfu be much lower with 293 as I do not have the hek 293 pack at hand?
thom
yes, thank you. will the pfu be much lower with 293 as I do not have the hek 293 pack at hand?
thom
#4
loustau
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Posted 17 March 2005 - 04:03 AM
I think you might want to make sure that you have 293A cells, since those were immortalized by the E1A/E1B oncogenes and will then support the deficient adenovirus amplification.
I know someone tried for a long time with 293T cells, without success. I believe those don't express E1A and E1B, but the T antigen, so be careful.
Good luck!
I know someone tried for a long time with 293T cells, without success. I believe those don't express E1A and E1B, but the T antigen, so be careful.
Good luck!
