Do all 293 cells contain an adenovirus?
Posted 16 March 2005 - 01:27 AM
Posted 16 March 2005 - 01:40 AM
try a search in the ATCC database. http://www.atcc.org/ (search in cell biology and "name"=293
But you're right, 293 cells (from kidney contains either adenovirus 5 sequnces or SV40 sequences or SV40/CMV...
But for virus amplification, i woul youse Hek 293 PACK cells also known as phoenix helper cells....
Did i answer your question?
Posted 16 March 2005 - 04:57 AM
yes, thank you. will the pfu be much lower with 293 as I do not have the hek 293 pack at hand?
Posted 17 March 2005 - 04:03 AM
I know someone tried for a long time with 293T cells, without success. I believe those don't express E1A and E1B, but the T antigen, so be careful.