Nuclear protein extract (Scrape or Trypsinize)?
Posted 15 March 2005 - 03:36 PM
Posted 16 March 2005 - 01:11 AM
i've never made a rigourous comparison of the two methods. But i would say that both methods are ok.
I choose the method regarding the number of plates i have.Till 20 plates, i scratch cells but more than 20 plates i prefer trypsin method that is quicker.
Obviously, scratching a 6 24 96 well-plate is quite impossible.
Edited by fred_33, 16 March 2005 - 07:03 AM.