southern hybridization temperature
Posted 14 March 2005 - 07:38 PM
now i wanna pursue a low stringency southern hybridizaion. the probe is ~1kb and with nearly only 70% homology with the target DNA.
how can i decide the hybridization temperature?
Posted 15 March 2005 - 02:03 AM
as you get only 70%homology with your target, i would first try a room temperature assay...
Posted 15 March 2005 - 03:33 AM
but RT? can the double strand denature and accomplish hybridization successfully in such low temperature?
and do you mean i have to try a temperature grads?